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Product Info Summary
| SKU: | M02561-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Monkey, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8]
View all GRP75/HSPA9B/Mortalin Antibodies
SKU/Catalog Number
M02561-1
Size
100 µl
Description
Boster Bio HSPA9 mouse monoclonal antibody, clone OTI9F8 (formerly 9F8). Catalog# M02561-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Monkey, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02561-1)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI9F8
Isotype
IgG2b
Immunogen
Full length human recombinant protein of human HSPA9 (NP_004125) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02561-1 is reactive to HSPA9 in Dog, Human, Monkey, Mouse, Rat
Applications
M02561-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Observed Molecular Weight
74 kDa
Calculated molecular weight
73.68kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Validation Images & Assay Conditions
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-HSPA9 mouse monoclonal antibody (M02561-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9.
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Immunofluorescent staining of HepG2 cells using anti-HSPA9 mouse monoclonal antibody (M02561-1).
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSPA9 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSPA9.
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Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-HSPA9 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-HSPA9 monoclonal antibody (1:200).
Protein Target Info & Infographic
Gene/Protein Information For HSPA9 (Source: Uniprot.org, NCBI)
Gene Name
HSPA9
Full Name
Stress-70 protein, mitochondrial
Weight
73.68kDa
Superfamily
heat shock protein 70 family
Alternative Names
75 kDa glucose-regulated protein; CSA; GRP75; GRP-75; GRP75mthsp75; Heat shock 70 kDa protein 9; heat shock 70kD protein 9B; heat shock 70kDa protein 9 (mortalin); heat shock 70kDa protein 9B (mortalin-2); HSPA9; HSPA9B; HSPA9BMGC4500; Mortalin; mortalin, perinuclear; Mortalin-2; MOT; MOT2; mot-2; MTHSP75; p66-mortalin; PBP74; PBP74CSA; Peptide-binding protein 74; stress-70 protein, mitochondrial HSPA9 CRP40, CSA, EVPLS, GRP-75, GRP75, HEL-S-124mB, MOT, MOT2, MTHSP75, PBP74, SAAN, SIDBA4, HSPA9 heat shock protein family A (Hsp70) member 9 stress-70 protein, mitochondrial|75 kDa glucose-regulated protein|catecholamine-regulated protein 40|epididymis secretory sperm binding protein Li 124m|heat shock 70kD protein 9B|heat shock 70kDa protein 9 (mortalin)|mortalin, perinuclear|mortalin-2|mortalin2|p66-mortalin|peptide-binding protein 74
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA9, check out the HSPA9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Grp75 (HSPA9) Mouse Monoclonal Antibody [Clone ID: OTI9F8] (M02561-1)
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